Detection of Aflatoxins from Maize Samples Using Ferric Chloride

Journal of Contemporary Chemistry

Published : 2022-10-31


Page Views: 225

open-end-wrenchArticle Tools PDF

Author: Eugine Mukhaye


Enzyme naringinase is a metabolite produced by a fungus called Aspergillus flavus and it changes colour from yellow to brick red when reacted with ferric chloride. Structurally, this enzyme is only two fewer carbon atoms compared to aflatoxins and it is produced by the same fungus that produces aflatoxins. Due to this structural relationship, the research borrowed the idea behind the colour change that occurs when the enzyme naringinase reacts with ferric chloride to give a notable colour change. The current methods for detecting aflatoxins are expensive and time-consuming and some of them such as thin-layer chromatography (TLC) are less sensitive and use carcinogenic reagents. The main aim of this research was to develop a fast, economical and sensitive assay for the detection of aflatoxin in maize. The isolation of aflatoxins from the maize sample was done through a direct method using 70% methanol. The screening of the presence of aflatoxin was done using an enzyme-linked immunosorbent assay (ELISA) test. A chemical test was later conducted on aflatoxins using 1% ferric chloride to give a brick-red colour. The concentration of this colour determined the quantity of aflatoxin. This study is relevant because aflatoxin is a killer toxicant and it also reduces the productivity of our livestock and crops. Therefore, there is a need for early detection of aflatoxin before it spreads to a wide range and causes further harm. Thus, using ferric chloride as a method for detecting aflatoxins is convenient because it is a rapid, simple and sensitive method compared to other methods such as thin-layer chromatography.

Keywords: Aflatoxins, Thin Layer Chromatography, Optical Density, ELISA, Parts per billion.


Mukhaye, E. (2022). Detection of Aflatoxins from Maize Samples Using Ferric Chloride. Journal of Contemporary Chemistry, Volume 1, Issue 1, P. 1 - 11.

About Us

GMP Journals is an open–access journal that accepts multidisciplinary articles committed to promoting research in the fields of humanities and social sciences, education, science and technology among others. It encourages scientific research by publishing the researched outcome in vast areas through the open-access model. It is aimed at the development and rapid dissemination of scientific knowledge to the global community without any restrictions.

Journal Info

  1. ISSN Print: 2788-9939
  2. ISSN Online: 2788-9947
  3. Journal DOI:
  4. Digital Archiving: Internet Archive, Zenodo


  1. DataCite
  2. Google Scholar
  3. OpenAIRE
  4. Zenodo

For Authors

  1. Article Sample
  2. Call for Papers
  3. Guidelines
  4. Peer Review

Useful Links

  1. Articles
  2. Books
  3. Contact Us
  4. Editors
  5. Mobile App
  6. Newsletters
  7. Privacy policy
  8. Free Certificate Course

Creative Commons License
Journals distribution is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.